J Korean Neurol Assoc > Volume 20(4); 2002 > Article

Journal of the Korean Neurological Association 2002;20(4): 385-393.

6 -HydroxydopamIne 을 이용한 편측 파킨슨 흰쥐 모델에서 선조체의 유전체 변화

김병조, 박건우 김 현 이대희

고려대학교 의과대학 신경과학교실, 해부학교실

"Genomic Changes in the Striatum of Unilateral 6-hydroxydopamine Lesioned Parkinson Rat Model"

Byung Jo Kim

Department of Neurology and Anatomy, Korea University School of Medicine

Abstract

"Background : Parkinson ’s disease is the primary degenerative disease characterized by rigidity, bradykinesia and resting tremor. Even though dopaminergic cell loss of the substantia nigra compacta is a main pathogenesis of the Parkinson ’s disease, the striatal dysfuntion is major pathophysiology of pakinsonian symptoms. Methods : Since gene expression profile can explain the symptomatic varieties of Parkinson ’s disease, gene expression was investigated in the striatum of rat brain after lesioning of unilateral substantia nigra compacta with 6-hydroxydopamine using cDNA microarray technique for the first time. The expression patterns of 5,200 rat brain cDNAs were screened and clustered according to the function of gene. The expression patterns of enkephaline and substance-P mRNA were also studied for validation of animal preparation. Results : Various genes involved in apoptosis, cytokines. Cytoskeletal molecules, neu-rotrophic factors, receptors, intracellular Ca 2+ metabolism, signal transduction, stress protein, cell cycle regulator pro-tein, and expressed sequence tags(EST) have shown significant expression changes. Conclusions : These gene expres-sions provide the global assessment of the processes involved in secondary change of striatum afforded by dopaminer-gic denervation at molecular levels.Key Words : Parkinson ’s disease, Microarray, 6-hydroxydopamine, Striatum"