Chronological Changes in the Rotational Behavior in Response to Apomorphine Administration in 6-Hydroxydopamine Parkinsonian Rat

J Korean Neurol Assoc > Volume 17(1); 1999 > Article

Journal of the Korean Neurological Association 1999;17(1): 117-121.

6-hydroxydopamine 파킨슨씨병 모델에서 apomorphine의 투여에 따른 회전운동 변화의 시간적 추이

홍근식, 김병곤·전범석·윤병우·이광우·노재규·이상복·명호진

서울대학교 의과대학 신경과학교실

Chronological Changes in the Rotational Behavior in Response to Apomorphine Administration in 6-Hydroxydopamine Parkinsonian Rat

Keun Sik Hong, Byung Gon Kim, Beom S. Jeon, Byung-Woo Yoon, Kwang-Woo Lee, Jae-Kyu Roh, Sang-Bok Lee, Ho-Jin Myung

Department of Neurology, College of Medicine, Seoul National University, 28, Yongon-dong, Chongno-Gu, Seoul, Korea, 110-744

Abstract

Background : Apomorphine-induced rotational behavior of unilateral 6-hydroxydopamine (OHDA) lesioned rat is widely used to develop anti-Parkinsonian treatments including drugs, neuroprotective therapy, and neural graft. Time course of changes in rotational behavior after lesioning, however, has not been fully elucidated. The aim of this study was to observe the chronological changes in the rotational response and to find the optimal period when this model is used for investigation of various therapies.
Methods: 6-OHDA was stereotaxically delivered to the unilateral substantia nigra in 13 rats. Rotational responses to apomorphine administrations were counted in the rotomotor on 2, 4, 8, 12, and 14 weeks after lesioning.
Results: The total turns for two hours increased continuously up to eight weeks, and then plateaued.
Conclusions: Apomorphine-induced rotations increase until eight weeks after 6-OHDA lesioning. Therefore, this Parkinsonian model should be used at least eight weeks after lesioning. Even though priming was not excluded as an explanation in the experiment, we reason that progressive degeneration of dopaminergic neurons may explain the chronological changes in rotational behavior. Key Words: 6-hydroxydopamine, Rotational behavior, Apomorphine