Protective Effect of Ginsenoside Rb1 and Rg1 Against β
Amyloid(25-35)-Induced Neurotoxicity on B103 cells

J Korean Neurol Assoc > Volume 17(5); 1999 > Article

Journal of the Korean Neurological Association 1999;17(5): 688-693.

B103 세포에서 베타아밀로이드(25-35)독성에 대한 Ginsenoside Rb1 과 Rg1 의 보호효과

이은아, 주인수*·허 균 ·묵인희

아주대학교 의과대학 신경과학교실

Protective Effect of Ginsenoside Rb1 and Rg1 Against β Amyloid(25-35)-Induced Neurotoxicity on B103 cells

Eun Ah Lee, M.D., In Soo Joo, M.D., Kyoon Huh, M.D., Inhee Mook, M.D.

Department of Neurology, School of Medicine, Ajou University

Abstract

Background : Ginseng extracts, known to enhance bodily functions including learning and memory, were reported to have in vitro neuroprotective activity in vitro. Here We demonstrate the possible therapeutic effects of ginsenosides on the cell culture model of Alzheimer’s Disease (AD). We tested whether Rb1 or Rg1 , major components of ginseng saphonins, protects neuronal cells from the toxic effect of ß-amyloid (Aß), which is regarded to be the main neurotoxic substrate in the AD. Methods : B103 cells, rat brain-derived neuronal cells, were cultured and the extent of neuropro-tective effects of ginsenosides on the cytotoxicity induced by exogenous Aß25-35 was were measured by MTT assay. Results : Treatment of Rb1 and Rg1 at various concentrations (l0nM, 50nM, and 1μM, respectively) in B103 cells did not show any dose-dependent neurotoxic effects. Rg1 (1μM) significantly blocked the neurotoxic effect of Aß2 5 - 3 5 (50μM)(P<0.05). Rb1 at concentration of 1μM also had some neuroprotective effects, but not as effective as Rg1 . These neuroprotective effects are comparable to the one of estrogen (1.8nM). Conclusions : This experiment suggests the potential beneficial effects of ginseng in the treatment of AD. J Kor Neurol Ass 17(5):688~693, 1999 Key Words : Alzheimer’s disease, ß-amyloid toxicity, Saphonin(Rb1 , Rg1 ) Protective effect