Normal Values of the Regional Cerebral Blood Flow (rCBF) in Rat Using Digital Autoradiographic Method

J Korean Neurol Assoc > Volume 9(4); 1991 > Article

Journal of the Korean Neurological Association 1991;9(4): 391-396.

계수형 자가방사기록법을 이용한 백서국소뇌혈류의 정상치

윤병우, 홍승봉,노재규

서울대학교 신경과.

Normal Values of the Regional Cerebral Blood Flow (rCBF) in Rat Using Digital Autoradiographic Method

Dep. Of Neurology, University

Abstract

To get the normal values of regional cerebral blood flow (rCBF) in rat brain in our laboratory, we used the digital autoradiographic method with C-14 iodoantipyrine (IAP) in 8 Wistar rats. The rats were anesthetized with 1.5% halothane during the experimental procedure. Right femoral artery and vein were cannulated for blood pressure monitoring. Arterial sampling - and injection of radiotracer. Fifty uCi of C-14 IAP was constantly injected over 30 seconds using infusion pump and 10 to 15 arterial samples were obtained with micropipettes At the end of 30 seconds the brain was removed and freezed in liquid nitrogen. Coronal sections of the brain were exposed to film and the images were analyzed by computer-based digital analyzer system. Values of the regional cerebral blood flow in ml/l00g brain/min were as follows. Frontal cortex, 110.7+31.0; parietal cortex, 120.9+31.0; temporal cortex, 123.4+30.5; occipital cortex, 111.2+31.; cingulate cortex, 112.8+32.4; pyriform cortex, 1043+33.8; entorhinal cortex, 114.5+40.9; corpus callosum, 55.2+17.2; caudatoputamen 105.3+ 39.8, thalamus, 114.6+35.0; Cal region of hippocanpus, 79.4+:33.3; CA2. 80.2+34.9; CA3, 83.4+37.2; dentate gyrus, 80.0+37.1. These normal values of rCBF in rat brain may be used as a standard index for future experimental researches on therapy and pathophysiology of cerebral ischemia using autoradiographic measurement of rCBF.