The Effects of (-)-Epigallocatechin Gallate on Rat Hippocampal Organotypic Slice Cultures Treated with the 1-42 β-amyloid Protein

J Korean Neurol Assoc > Volume 23(6); 2005 > Article

Journal of the Korean Neurological Association 2005;23(6): 806-813.

쥐 해마 절편배양에서 베타-아밀로이드 1-42분획 독성에 대한 (-)-Epigallocatechin Gallate의 효과

중앙대학교 의과대학 신경과학교실

The Effects of (-)-Epigallocatechin Gallate on Rat Hippocampal Organotypic Slice Cultures Treated with the 1-42 β-amyloid Protein

Young-Chul Youn

Department of Neurology, Chung-Ang University College of Medicine, Seoul, Korea

Abstract

Background: Considerable evidences suggest that the β-amyloid acts as a neurotoxin, and the epigallocatechin-3- gallate (EGCG) has the anti-inflammatory and anti-oxidant properties. The purpose of this study was to investigate whether the EGCG reduces the death of the cultured hippocampal tissues exposed to the β-amyloid 1-42 fragments (Aβ1-42). Method: We cultured the hippocampus of postnatal 7 days old Sprague-Dawley rat into slices of 450 μm. The tissue slices had been exposed with 100 μM Aβ1-42 at an interval of 3 days since 12 DIV (days in vitro). Following co-treatment of the tissue with 10 μM EGCG and Aβ1-42, we evaluated EGCG effect on Aβ1-42 induced neurotoxicity by measuring the expression of Bcl-2 and NeuN protein and by morphological observation of the hippocampus slice cultures with propidium iodide (PI) and bromodeoxyuridine (BrdU) staining.
Results: The EGCG exerted a significant role in restoration of NeuN protein expression inhibited by Aβ1-42, showed inhibitory effects fluorescence in PI stained tissues, and increased the anti-BrdU stained cell.
Conclusions: 10 μM EGCG reduced the Aβ1-42 induced neurotoxicity of the hippocampus slice culture. KeyWords:Alzheimer's disease, Amyloid, Hippocampus, Epigallocatechin gallate