| 백서 뇌출혈 모델에서 내인성 신경줄기세포의 증식 |
| 강규식, 정상욱b 주 건c 정근화 김만호 노재규 |
| 서울대학교병원 신경과학교실, 임상의학연구소 줄기세포연구센터 뇌졸중-줄기세포 실험실,
서울대학교 의과대학 의학연구원 신경과학 연구소 뇌신경과학 과정
아산정신병원 신경과a, 인제대학교 일산백병원 신경과b, 국립서울병원 알콜약물연구센터c |
| Endogenous Neural Stem Cell Proliferation after Intracerebral Hemorrhage in Rat Model |
| Kyusik Kang |
| Stroke & Neural Stem cell Laboratory in Clinical Research Institute, Stem Cell Research Center,
Department of Neurology, Seoul National University Hospital, Program in Neuroscience, Neuroscience Research Institute of SNUMRC, Seoul National University;
Department of Neurology, Asan Mental Hospitala, Asan;
Department of Neurology, Inje University Ilsan-Paik Hospitalb, Ilsan;
Center for Alcohol and Drug Addiction Research, Seoul National Hospitalc, Seoul, Korea |
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| Abstract |
Background: Focal and global ischemia was shown to be potent in inducing neurogenesis in the subventricular zone (SVZ). However, it is unknown whether these new neurons can replace those lost following damage, such as intracerebral hemorrhage (ICH). We tested the hypothesis that ICH leads to an increase of cell proliferation in the SVZ and that the new neurons migrate into the damaged area of the hemorrhagic striatum.
Methods: Experimental ICH was induced by an intrastriatal administration of collagenase in adult rats. Proliferating cells were labeled with intraperitoneal injections of 5-bromo-2'-deoxyuridine-5'-monophosphate (BrdU) over 3-day periods before sacrificing the animals, 0, 6, 9, 12 or 15 days after ICH. BrdU＋ cells in the dorsolateral SVZ and the striatum were counted in three coronal sections per animal.
Results: BrdU＋ cells were found in the bilateral SVZs, the third ventricle, and ipsilateral cortex near the needle insertion site. The number of BrdU＋ cells in the damaged striatum and the ipsilateral SVZ reached maximum counts 9 days after ICH. The BrdU＋ cells were double-labeled with a neuronal marker, neuronal nuclear antigen (NeuN), and a glial marker, the glial fibrillary acidic protein (GFAP), in the hemorrhagic hemisphere. The percentage of BrdU＋ cells that expressed NeuN and GFAP was ?13% and ?72%, respectively.
Conclusions: These results suggest new evidence that endogenous neural stem cells are activated in the dorsolateral SVZ after ICH, and that the newly formed cells play a role for ICH-induced neurogenesis in what may be an adaptive process that contributes to recovery after a hemorrhagic stroke.KeyWords:Neural stem cell, Neurogenesis, Intracerebral hemorrhage |
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