J Korean Neurol Assoc > Volume 21(4); 2003 > Article

Journal of the Korean Neurological Association 2003;21(4): 387-391.

"일시적 국소허혈에 의해 유발된 흰쥐 대뇌 신경세포 손상에 대한 EGCG(epigallocatechin gallate)의 신경보호 효과"

최영빈 , 박정욱 한시령 이광수 김범생

가톨릭대학교 의과대학 신경과학교실

"Protective Effects of the Green Tea Polyphenol(-)-epigallocatechin Gallate Against Ischemia Reperfusion Injury Induced by Middle Cerebral Artery Occlusion in Rats"

Young-Bin Choi

Department of Neurology, College of Medicine, The Catholic University of Korea

Abstract

"Background: EGCG(epigallocatechin gallate), a major green tea extract, is a potent free radical scavenger which has been shown to reduce free radical-induced lipid peroxidation. The purpose of this study was to examine whether EGCG reduces focal ischemia/reperfusion-induced brain injury in rats.
Methods: Male Wistar rats were anesthetized with ketamine and xylazine and subjected to 120 min of temporary middle cerebral artery occlusion by an intraluminal nylon suture coated with poly-L-lysine. The drug (EGCG, n=8) or vehicle (normal saline, n=8) was administered iv.(as a 50 mg/kg bolus) immediately after the onset of middle cerebral artery occlusion. Neurologic status was evaluated 2 hours after occlusion and 24 hours after. Twenty-four hours after ischemia, the brain was perfusion-fixated and the infarct volume was determined.
Results: EGCG significantly improved the neurological status at 24 hours after middle cerebral artery occlusion.(p<0.05), and reduced total infarct volumes (p<0.01).
Conclusions: These results demonstrate the neuroprotective effect of EGCG in a rat model of transient focal cerebral ischemia. Key Words: EGCG, Ischemia, Rat"