J Korean Neurol Assoc > Volume 21(3); 2003 > Article

Journal of the Korean Neurological Association 2003;21(3): 289-296.

"파킨슨병 흰쥐 동물모델에서 배아줄기세포 유래 및 태아중뇌 미분화신경세포 유래 도파민 신경세포의 치료 효과"

고현철 , 박장환 전범석이상훈

"한양대학교 의과대학 약리학교실.미생물학교실 *.생화학교실 .서울대학교 의과대학 신경과학교실"

"Efficacy of Embryonic Stem Cell-derived Dopaminergic Neurons and E12 Mesencephalic Neuronal-precursor Derived Dopaminergic Neurons as a Source for Transplantation in Parkinsonian Rats"

Hyun Chul Koh

"Department of Pharmacology, Microbiology*, Biochemistry ,College of Medicine, Hanyang University Department of Neurology, College of Medicine, Seoul National University"

Abstract

"Background: This study was performed to compare efficiency of embryonic stem (ES) cell-derived dopaminergic (DA) neurons from E12 mesencephalic neuronal-precursor derived DA neuron, and to assess the value of these neurons as a donor cells to transplant in Parkinson s disease.
Methods: ES cell-derived DA neurons from E12 mesencephalic neuronal-precursor derived DA neuron were transplanted in Parkinsonian rats and analyzed TH immunoreactivity and apomorphin-induced rotation test.
Results: Transplanting of ES cell derived DA neurons into the rat striatum caused gradual increase of expression of tyrosine hydroxylase (TH) immunoreactivity 6 weeks after transplant in host striatum. Rotation behavior was gradually on the decrease, 6 weeks after transplant of ES cell derived DA neurons. In case of E12 mesencephalic precursor derived DA neurons, the TH immunoreactivity expressed 6 weeks after transplant in stria-tum, and the behavioral restoration of DA-mediated motor asymmetry sustained gradually. However, transplanted ES cell derived DA neurons appeared in wide regions among the needle tract with a relatively high density of TH immunoreactivity in comparison to the E12 mesencephalic precursor DA neurons, and graft volume of ES cell derived DA neurons were larger than E12 mesencephalic precursor derived DA neuron. In addition, both cells did not appear cancerous change after transplantation.
Conclusions: These results suggest that ES cell is more efficient than E12 mes-encephalic precursor derived dopaminergic neuron and will supply efficient donor cells for transplantation in Parkinson s disease.Key Words: Embryonic stem cell, E12 mesencephalic precursor derived DA neuron, Parkinson s disease; Dopaminergic neuron transplantation"