J Korean Neurol Assoc > Volume 20(6); 2002 > Article

Journal of the Korean Neurological Association 2002;20(6): 682-693.

"산발성 근위축성측삭경화증 환자의 뇌에서 관찰한 신경원 및 신경교세포 PARP 면역활성도의 증가"

김승현 , "Jozsef I.Engelhardt 김주한 공 구 Stanley H.Appel"

"한양대학교 의과대학 신경과학교실,병리학교실, B a y l o r 의과대학 신경과학교실, Szeged 의과대학 신경과학교실"

"Increased Neuronal and Glial Poly (ADP-Ribose) Polymerase Immunoreactivity in the Brain of Sporadic Amyotrophic Lateral Sclerosis"

Seung Hyun. Kim, M.D.

"Department of Neurology, Pathology, College of Medicine, Hanyang University, Seoul, Korea Department of Neurology, Baylor College of Medicine, Houston, Texas, U.S.A. Department of Neurology, University of Szeged, Szeged, Hungary"

Abstract

"Background : Over activation of the DNA repairing enzyme, poly (ADP-ribose) polymerase (PARP) in response to oxidative damage of DNA appears to play a role in cellular death in neurodegenerative diseases. Previous data suggest-ed that PARP immunoreactivity (IR) was increased in the white and gray matter in spinal cord of the sporadic amy-otrophic lateral sclerosis (sALS), predominantly in cells with astroglial morphology. Methods : In the present study, we evaluated whether the PARP expression was present widespread in various regions of brain tissue including the motor cortex, parietal cortex and cerebellum. Results : By western blot, PARP-IR in motor cortex from sALS patients, com-pared to the same region from age-matched normal controls, was also significantly increased (p=0.006). Importantly, PARP-IR was also increased in the parietal cortex, and cerebellum of sALS patients compared to the controls, in regions that are usually clinically unaffected in ALS (p=0.043, p=0.035, respectively). In addition, increased PARP expression in ALS was more prominent compared to Alzheimer s brain. Immunohistochemistry revealed that PARP staining was more significant in the cortical neurons and in the subcortical white matter glial cells from sALS patients compared to normal controls and Alzheimer s disease. Conclusions : The data demonstrate that increase in PARP-IR is not limited only to the vulnerable motor cortex. Furthermore, PARP-IR is present in both cortical neuronal and subcorti-cal glial cells. The data suggest that widespread cellular stress on neuronal and glial cells is present in the brain of spo-radic ALS patients.Key Words : Poly (ADP-ribose) polymerase, Amyotrophic lateral sclerosis, Motor neurons, Glia, Alzheimer disease"