| 근위축성측삭경화증 환자의 면역글로불린이 PC12 세포의 세포내 칼슘 농도에 미치는 영향 |
| 김성훈, ·정승준‡·박경석†·김만호 ·김김전*·김현정 ·이광우 |
| 서울대학교 의과대학 신경과학교실,생리학교실*,서울 백병원 신경과†,단국대학교 의과대학 생리학교실‡ |
| Effects of the Immunoglobulins of Amyotrophic Lateral Sclerosis on Intracellular Calcium in PC12 Cells |
| Sung Hun Kim, M.D., Sung Jun Jung, M.D.‡, Kyung-Seok Park, M.D.†,Manho Kim, M.D., Jun Kim, M.D.*, Hyun-Jung Kim, M.D., Kwang-Woo Lee, M.D. |
| Department of Neurology and Physiology*, College of Medicine, Seoul National University Department of Neurology, Seoul Paik Hospital† Department of Physiology, College of Medicine, Dankook University‡ |
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| Abstract |
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Background : The motor neuronal death in amyotrophic lateral sclerosis (ALS) arise from several mechanisms including excitotoxicity, oxidative stress, immunologic or calcium dysregulation. Though the immunoglobulin mediated neuronal death has been implicated through changes of calcium influx, the direct evidence showing the increase of intracellular calcium level is rare. The purpose of our study is to directly measure the calcium in cultured neurons treat-ed with immunoglobulins from ALS patients to further understand the role of calcium in motor neuron degeneration. Methods : Immunoglobins purified from serum in patients with ALS (n=8) and in control (n=8) were incubated for 24 hours in PC12 cell line. The intracellular calcium level was measured by fluorometry using calcium chelating dye, fura-2. High concentration of potassium chloride were used to evoke calcium influx, and L-type calcium channel current were measured using nicardipine, the L-type specific calcium channel blocker. Results : Basal level of intracellular cal ?cium was higher in the cells treated with immunoglobulins from ALS. However, the calcium increase evoked by 30 mM or 140 mM potassium chloride were lower in ALS than in control. The calcium increase through L-type calcium channel in ALS group was not significantly different from control. Conclusions ; Considering the proposed role of the calcium in neuronal degeneration, present data suggest increased intracellular calcium is important in neurodegenerative mechanism caused by ALS immunoglobulins.J Korean Neurol Assoc 18(6):728~734, 2000Key Words : Amyotrophic lateral sclerosis, Calcium, Immunoglobulins |
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