The Effect of U-74389G and MK-801 on the Size of
Brain Infarction in the Transient Focal
Ischemia-Reperfusion Rat Model

J Korean Neurol Assoc > Volume 17(1); 1999 > Article

Journal of the Korean Neurological Association 1999;17(1): 138-145.

백서의 일과성 국소허혈-재관류 모델에서 U-74389G와 MK-801이 뇌경색 크기에 미치는 효과

김재일, 김종민*, 양대웅, 이근호, 윤병우*, 노재규*, 이상복

단국대학교 의과대학 신경과학교실, 서울대학교 의과대학 신경과학교실

The Effect of U-74389G and MK-801 on the Size of Brain Infarction in the Transient Focal Ischemia-Reperfusion Rat Model

Jae-Il Kim, Jong-Min Kim*, Dae-Woong Yang, Geun-Ho Lee, Byung-Woo Yoon*, Jae-Kyu Roh*, Sang-Bok Lee*

Department of Neurology. College of Medicine, Dankook University San-29 Anseo-dong Cheonan, Choongnam 330-714 Korea

Abstract

Background : There has been considerable progression in laboratory investigations of novel therapeutic approaches aimed at protecting the brain parenchyma in the setting of the acute ischemia. The zone of ischemia surrounding an area of infarction provides a target for neuroprotective agents. MK-801 is a well known non-competitive NMDA receptor antagonist and has a neuroprotective effect to ischemic penumbra zone. U-74389G is a compound of Lazaroid(21-Aminosteroid) and a free radical scavenger which has been investigated to a neuroprotective effect to ischemic penumbra zone. Methods : The protective effects of single or combined pretreatment of U-74389G and MK-801 on the focal cerebral ischemia in the Sprague-Dawley rats were evaluated. The size of infarction after 2 hours occlusion-4 hours reperfusion of the left middle cerebral artery with modified Longa method was measured. The rats were given 3mg/Kg I.v. of MK-801 and U-74389G or 0.5ml of normal saline 30 minutes before middle cerebral artery occlusion. The size of infarction was described as the volume. Results : U-74389G, MK-801, and combined U-74389G and MK-801 pretreatment reduced significantly the volume of infarction 55%, 64%, 24%, respectively compared with saline pretreatment (p<0.05) and the combined U-74389G and MK-801 pretreatment reduced significantly the volume of infarcted area compared with MK-801 or U-74389G pretreatment, 44%, 37%, respectively(p<0.05). There was no significant change of infarction volume between U-74389G and MK-801 pretreatment(p>0.05). Conclusions : These results show that single or combined pretreatment of U-74389G and MK-801 before 30 minutes of occlusion significantly reduced the volume of infarction compared with the control group in the focal cerebral occlusion-reperfusion of rats. And the combined pretreatment of U-74389G and MK-801 before 30 minutes of occlusion significantly reduced the volume of infarction compared with the single pretreatment of MK-801 or U-74389G in the focal cerebral occlusion- reperfusion of rats. Key words : Focal cerebral ischemia, U-74389G, MK-801