Monoamine oxidase B gene and Cytochrome P450 2D6
gene polymorphism in sporadic Korean 
Parkinson's disease

J Korean Neurol Assoc > Volume 17(1); 1999 > Article

Journal of the Korean Neurological Association 1999;17(1): 63-71.

한국인 파킨슨병에서 Monoamine oxidase B 유전자와 Cytochrome P450 2D6 유전자의 다형성

이용희, 임정근* 이상도* 이영재

한동대학교 선린병원 신경과 계명대학교 의과대학 신경과학 교실* 및 의과학 연구소

Monoamine oxidase B gene and Cytochrome P450 2D6 gene polymorphism in sporadic Korean Parkinson's disease

Yong-Hee Lee,M.D., Jeong-Geun Lim,M.D.*, Sang-Doe Yi,M.D.*, Young-Jae Lee,Ph.D.

Department of Neurology,College of Medicine Keimyung University,Dongsan Hospital, 194 Dongsan Dong,Taegu,Korea

Abstract

Background : Epidemiological studies have identified that positive family history and frequent exposures to environmental toxins such as 1-methyl-4-phenyl-1,2,3,6- tetrahydropyridine (MPTP) are of prime causative factors for PD. These toxins are mainly metabolized by MAO-B and CYP2D6. Thus, an individual with inherited defect in xenobiotic metabolism could have a higher susceptibility to PD. We performed this study to investigate a possible allelic association of MAO-B and CYP2D6 known to be involved in metabolism of dopamine and other drugs such as debrisoquine in PD. Methods : We studied polymorphism of MAO-B and CYP2D6 genes in 69 sporadic idiopathic PD patients (31 males and 38 females) and 41 age-matched healthy control (20 males and 21 females) using genomic DNA extracted from peripheral blood white cell with polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP). Results : There were eight different alleles of various numbers of GT repeats within the second intron of MAO-B. The frequency of (GT)20 allele was the highest (44.7%) in PD, while the frequencies of (GT)14 allele and (GT)19 allele were the highest in control groups. Furthermore, the odds ratios of (GT)16 allele and (GT)20 allele were 4.93 (95% confidence interval 0.6-107.63) and 6.15 (95% confidence interval; 2.52-15.51), respectively, suggesting a higher susceptibility to PD in (GT)20 allelic group (p<0.001). Polymorphism of CYP2D6 was also examined by PCR amplification followed by digestion with restriction enzymes. However, we were unable to identify G to A substitution at the junction of intron 3 and exon 4 nor base pair deletion in exon 5 from PD and control groups, which have been reported previously. Conclusions : These results suggest that the MAO-B gene polymorphism could serve as a determinant of genetic susceptibility to PD at least in Korean population. But the susceptibility may not be directly associated with polymorphism of CYP2D gene examined in this study. Key Words : Parkinson's disease, Monoamine oxidase B, CYP2D6, Gene polymorphism