The Effects of Unilateral Dopaminergic Deafferentation
on the Expression of mRNAs of Neurotrophic Factors

J Korean Neurol Assoc > Volume 13(3); 1995 > Article

Journal of the Korean Neurological Association 1995;13(3): 425-437.

흰쥐 편측 도파민성 신경계 소실이 신경영양성 인자들의 유전자 ?발현에 미치는 영향

박민규, 김현,이대희

고려대학교 신경과,해부학교실

The Effects of Unilateral Dopaminergic Deafferentation on the Expression of mRNAs of Neurotrophic Factors

Min Kyu Park, M.D., Hyun Kim, MD., Dae Hie Lee , M.D.

Department of Neurology, and Anatomy, College of Medicine, Korea University

Abstract

Recently protective and supportive functions of neurotrophic factors on dopaminergic neurons have been reported. In this study, in situ hybridization histochemistry with ""S-labeled oligonucleotide probes for brain-derived factor (BDNF) and neurotrophin-3 (NT-3) mRNAs was performed to determine the effect of unilateral deafferentation of midbrain dopaminergic cells with 6-hydroxydopam'me (6-OHDA) on the expression of niRNAs of the above neurotrophic factors in the hippocampal areas. The deafferentation of midbrain dopaminergic cells induced changes of expression of BDNF mRNAs and NT-3 mRNAs. Although the reduction of NT-3 MRNA is limited to dentate gyrus of the lesion side, the induction of BDNF MRNA was observed in the lesion side firstly and then showed in the contralateral side conseqently. These results support the suggestion that these neurotrophic factors may protect or support dopaminergic neurons. In addition, these data propose the possibility that neurotrophic factors may be related with degenerative diseases such as Parkinson's disease .