| 신생흰쥐의 실험적 뇌경색- 저산소에 대한 니트로글리세린과 페니토닌의 효과 |
| 김영백, 김한식, 박 관, 안영환, 민병국, 황성남, 석종식, 최덕영, 권오상 |
| 중앙대학교 신경외과. 신경과 |
| The Neuroprotective Effects of Transdermal Nitroglycerin and Intraperitoneal Phenytoin on Ischemic-Hypoxic Injury in ?Developing Rat Brains |
| Young Baeg Kim, M.D., Han Sik Kim, M.D., Kwan Park, M.D., Young Hwan Ahn, M.D., Byung Kook Min, M.D., Sung Nam Hwang, M.D., Jong Sik Suk, M.D., Duck Young Choi, M.D., Oh Sang Kwon, M.D. |
| Department of Neurology, and Neurosurgery College of Medicine, Chung-Ang University |
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| Abstract |
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The goal of this study was to evaluate neuroprotective effects of nitroglycerin and phenytom using in the developing rat brains Seven days old Sprague-Dawley rats underwent right carotid ligation followed by 8% 02 exposure (humidified, balanced with mtrogen) for 3 hours under the halothane anesthesia (control group, N=58). Body temperature of rats was accurately controlled before and during hypoxia. In nitroglycerin treated group (N=33), nitroglycerin was delivered chronically via patches (in escalating doses to 4mg/kg/hr) for 36hrs before and 48hrs postischemia. Phenytoin treated group(N=17) received intraperitoneal phenytoin(30mg/kg) before ischemia. Combined treated group(N=31) with nitroglycerin and phenytoin received two compounds with same method as above. Animals. Sacrificed one week later and histopathological evaluation for ischernic neuronal damage were conducted employing hemat,oxylin eosin staining and measurements of the hemispheric weight difference.
Phenytoin was effective in reducing neuronal damage in terms of weight
comparison(24+2.4%, atrophy in control vs. 5+2.9% in phnytoin group, p<0.001) and
ischemic changes in hippocampal area(p<0.05 in CA1, CA2, and CA3 area).
Transdermal nitroglycerin did not show any beneficial effects compared with control
group(23+3.0% in nitroglycerin group vs. 24+2.5% in control). Combined treated group
showed neuroprotection on weight comparison(24+2.4% in control vs 4+1.8% in combined
group, p<0.001) and ischemic changes in hippocampal area(p |
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