The effects of kainate-induced Seizure on the Opioid Peptide Gene? Expression in Rat Hippocampus

J Korean Neurol Assoc > Volume 12(4); 1994 > Article

Journal of the Korean Neurological Association 1994;12(4): 614-622.

흰쥐의 해마에서 Kainate 유발 경련이 오피오이드 펩티드 유전자 ?발현에 미치는 영향

박건우, 박민규,김병조,고성범,강윤희,이대희

고려대학교 신경과, 해부학.

The effects of kainate-induced Seizure on the Opioid Peptide Gene? Expression in Rat Hippocampus

Kun Woo Park, M.D., Min Kyu Park, M.D., Byung Jo Kim, M.D., Sung Bum Ko, M.D., Youn Hee Kang, M.D., Dae Hie Lee, M.D.

Department of Neurology, College of Medicine, Korea University, Department of Anatomy, College of Medicine, Korea University

Abstract

In the rat hippocampal formation, the time-course and dose-response of the expression of enkephalin and dynorphin gene were examined after kainate (KA) treatment with in situ hybridization histochemistry. The KA induced enkephalin and dynorphin mRNA expression in hippocampus occurred mainly in the dentate gyrus. The enkephalin mRNA expression appeared at 3hour after KA injection, increased dramatically at 6hour, and then decreses. At 24hour after KA injection, the expression of enkephalin mRNA disappeared. The dynorphin mRNA expression appeared at once after injection and increased dramatically at 3hour. Unexpectedly at 6hour after injection, the expression was decreased, and then increased less than the 3hour expression. The increased pattern persisted to 24hour after injection. Unexpected result was also encounted in the experiment of KA dose-response of enkephalin mRNA and dynorphin mRNA. In the hippocampal formation, in contrast with other areas, low dosage (8mg/kg) of KA induced more significant expression of both genes than high dosage (16mg/kg) of KA.