Diagnosis of Tuberculous Meningitis by Enzyme-Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) ?in CSF

J Korean Neurol Assoc > Volume 10(1); 1992 > Article

Journal of the Korean Neurological Association 1992;10(1): 1-7.

결핵성 뇌막염 진단에서 중합효서 연쇄반응법(PCR)과 효소결합 면역분석법(ELISA)의 비교연구

김원주, 김윤중,박수철,이병인,윤경한,조상래,김주덕

연세대학교 신경과, 미생물학.

Diagnosis of Tuberculous Meningitis by Enzyme-Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction (PCR) ?in CSF

Won-Joo Kim, M.D., Yoon-Joong Kim, M.D., Soo-Chul Park kk, Byung-In Lee, M.D.

Department of Neurology, Department of Microbiology., College of Medicine, Yoensei University

Abstract

Cerebrospinal fluid from 27 patients with tuberculous meningitis and from appropriate control individuals were assayed for immunoglobulin G activity to tuberculin purified protein derivative (PPD) and Lipoarabinomannan-B (LAM-B) antigens by a'n enzyme-linked immunosorbent assay (ELISA), and polymerase chain reaction (PCR). A significant difference was found between the tuberculous meningitis group and control. The sensitivity and specificity of these tests in cerebrospinal fluid was 59.2% and 93.9% for PPD, 85.2% and 95.9% for LAM-B antigen, and 70.3% and 89.7% for PCR method. The ELISA method measuring IgG antibody to LAM-B in cerebrospinal fluid and PCR method were both reliable and complementary each other for early diagnosis of patient with tuberculous meningitis.