The Stereotaxic Injection of Kainic Acid into Corpus Striatum: Destruction of Neuron with Sparing Axon

J Korean Neurol Assoc > Volume 9(3); 1991 > Article

Journal of the Korean Neurological Association 1991;9(3): 315-322.

쥐 줄무늬체속으로의 Kainic acid 직접투여가 신경원에 미치는 형태학적 변화

안병철, 김두응,권오상,김경용,이원복,김동창

중앙대학교 신경과, 해부학.

The Stereotaxic Injection of Kainic Acid into Corpus Striatum: Destruction of Neuron with Sparing Axon

Byeong Cheol Ahn, M.D., Doo Eung Kim, M.D., Oh Sang Kwon, M.D., Kyung Yong Kim, M.D.*, Won Bok Lee, M.D.*, Dong Chang Kim, M.D.*

Dep. Of Neurology & Anatomy*, Chung Ang University

Abstract

The ultrastructure of the excitotoxic lesion similar to that occurring in the degenerative neuronal disease was produceby stereotaxic injections of 1 nmol and 10 mnol of kainic acid nto the corpus striatum of adult rat brain There were rnarked swellings in the neuronal dendrites at injected sites. Neurotubules and neurofilarnents were disrupted as and amorphous materials and scattered throughout the interior of distended dendrites. Internal cristae and membranes of mitochondria were destroyed with the loss of integrity of intracellular organelles. Disruption of cellular and nuclear membranes occurered in severe cases. But there was no apparent pathologic change in the other structure, ie, synapses, presynaptic and postsynaptic parts, axons and glial cells. The synapses between dendrites and axon terminals were not destroyed despite of marked distension of dendrites. The local administration of excitatory amino acid into the brain caused the destruction of dendrites and neuronal cell bodies, but axons and axon terminals were intact With the lapse of time, axons and axon terminals from the destroyed neuron degenerate Therelore stereotaxic injection of excitatory amino acid into the brain may provldes a method of investigating neuronal connectivity.