| 태생기 생쥐의 각 장기 발생 과정에서 phospholipase C와 Siah 유전자 단백의 발현에 관한 면역조직화학적 연구 |
| 박은미, 김성숙, 최경규, 박기덕, 서판길, 신회섭 |
| 이화여자대학교 의대 신경과, 울산대학교 의대 병리학과, 포항 공대 생명과학과 |
| The expression of phospholipase C and Siah gene product during murine fetal development by immunohistochemistry |
| Eun Mi Park, Sung Sook Kim, Kyoung Gyu choi, Kee Duck Park, Pann Ghill Suh, Hee Sub Shin |
| Department of Neurology, College of Medicine, Ewha Womans University, Department of Pathology, College of Medicine, University od Ulsan and Department of life science, Pohang Institute of Science and Technology |
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| Abstract |
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Recently identified second messenger molecules of signal transmission were derived from inositol phospholipid and several phospholipase C(PLC) isoenzyme were reconized. But the role of PLC in embryonal organ development, differentiation and growth is not described, yet. And, Siah gene of mouse is expressed at a higher level in a restricted number of sites during development. So, it is suggested that the Siah gene plays a significant role in development of vertebrates.Therefore, this study was aimed to investigate the role of PLC isoenzymes and Siah gene during murine fetal development by use of immunehistochemistry. The results are as follow :
1. PLC-β1 and PLC-γl are expressed apparently at early fetal stage, PLC-βl is
observed focally, whereas PLC-γ1 is diffusely expressed.
2. PLC-δ1 is not expressed during early and mid murine fetal age but strongly
expressed in placental tissue.
3. PLC-β1 is observed mainly in neural tissues including brain at mid fetal stage.
PLC-γ1 is expressed in entire organs, especially in liver tissue, and then their
expression is reduced after mid stage.
4. Siah gene product is significantly expressed in olfactory epithelium, retina, neuron of
brain and choroid plexus from mid fetal stage to late gestation.
These results suggest that PLC-βl play a important role in central neural development
and PLC-γ1 in entire orgaans, especially in liver development. The strong expression of
PLC-δl in placenta suggest its involvement in early development, indirectly. Siah gene
product expressed at a higher levels in olfactory epithelium, retina, neuron and choroid
plexus, so Siah gene may play a important role in these organ developments. |
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