Application of Proteomics and Protein Chip Analysis in the Diagnosis of Neurodegenerative Disorders

J Korean Neurol Assoc > Volume 21(6); 2003 > Article

Journal of the Korean Neurological Association 2003;21(6): 584-599.

퇴행성 뇌질환 진단을 위한 프로테오믹스와 단백질 칩 분석의 응용

류원정 , 최철희

이화여자대학교 대학원 분자생명과학부

Application of Proteomics and Protein Chip Analysis in the Diagnosis of Neurodegenerative Disorders

Wonjung Ryu

Division of Molecular Life Sciences and Center for Cell Signaling Research, Ewha Womans University, Seoul, Korea

Abstract

As we face an increase of the adult population suffering from dementia, a typical senile disorder, it is imperative to develop appropriate tools for early detection and differential diagnosis of dementia. Recently, proteomics techniques have been proposed to be used for diagnosis of neurodegenerative disorders by identifying numerous biological markers that are known to increase or decrease in the cerebrospinal fluid or serum of dementic patients. Protein chip analysis, one of the most important techniques of proteomics, is suggested to be useful for examining various modifications of proteins as the high throughput screening method using small volumes of precious samples in a short period. We describe here a list of biological markers, such as Aβ, APP, tau, ubiquitin, S100B, soluble IL-6 receptor, beta 2 micoglobulin and prostaglandin E2, proposing that these molecules can be used as biochemical markers of dementia. Therefore, we suggest that the proteomic approaches to analyze the amount and modifications of these proteins might be powerful tools for early detection and differential diagnosis of various neurodegenerative disorders as well as assessment of disease progress.Key Words: Neurodegenerative Disorders, Proteomics, Biomarker, Protein array analysis, Cerebrospinal fluid