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Journal of the Korean Neurological Association 2001;19(1): 36-44.
Kainate로 유발한 측두엽간질 모델에서 Glutamate 수용체 아형 유전자의 발현
김명규, 최성민 ·이승한 ·김병채 ·조기현 ·남상채*·이민철*
전남대학교 의과대학 신경과학교실,의과학연구소*
Glutamate Receptor Subunits Gene Expression in Kainate-induced Temporal Lobe Epilpsy Model
Myeong-Kyu Kim, M.D., Sung-Min Choi, M.D., Seung-Han Lee, M.D., Byeong-Chae Kim, M.D.,Ki-Hyun Cho, M.D., Sang-Chae Nam, Ph.D.*, Min-Chul Lee, M.D.*
Department of Neurology and Research Institute of Medical Science*,Chonnam National University Medical School
Background : There is considerable controversy about the exact molecular mechanisms of excitatory amino acid receptors in epileptogenesis. Methods : We used in situ hybridization to examine the hybridization density (HD) of n-methyl- D-aspartic acid receptor type 1 (NMDAR-1) and alpha-amino-3-hydroxy -5-methyl-4-isoxazole-propionate (AMPA) receptor type 2 (GluR-2) mRNA, in the hippocampus obtained from the kainic acid (KA)-induced status epilep-ticus (SE) model. Some Sprague-Dawley rats were injected with KA (10 mg/Kg; I.p.), and others with MK-801 (4 mg/kg) 20 minutes prior to KA. The rats were allowed to have 4-hour SE and were killed at 8 hours or 4 weeks after KA or MK-801/KA injection. HD of NMDAR-1 and GluR-2 mRNA in subfields of the hippocampus was measured by an image analysis system. Results : A typical neuropathological finding of hippocampal sclerosis and spontaneous repetitive seizures (SRS) were observed in the KA injected rats, but not in the MK-801 pretreated rats, killed at 4 weeks. Compared with controls, the rats killed at 8 hours after KA showed increased CA1, CA2, and CA3 NMDAR-1 HD, and stratum granulosum (SG) GluR-2 HD. The increase of NMDAR-1, not GluR-2, HD was blocked effectively by MK-801. The increase of SG GluR-2 HD remained until 4 weeks after the KA injection. Conclusions : Not only the NMDAR-1activa-tionbut also the GluR-2 activation is an important factor in delaying hippocampal neuronal loss and epileptogenesis.J Korean Neurol Assoc 19(1):36~44, 2001Key Words : Temporal lobe epilepsy, Hippocampal sclerosis, SE, NMDA, AMPA, Kainic acid