| 일과성 전뇌허혈에서 허혈 지속 시간과 체온이
해마의 Bax/Bcl-2 발현에 미치는 영향 |
| 이용석, 박성호 ·윤병우*·노재규* |
| 서울특별시립 보라매병원 신경과,서울대학교 의과대학 신경과학교실* |
| The Effect of Duration of Ischemia and Body Temperature on the
Expression of Bax/Bcl-2 in Transient Global Ischemia |
| Yong-Seok Lee, M.D., Seong-Ho Park, M.D., Byung-Woo Yoon, M.D.*, Jae-Kyu Roh, M.D.* |
| Department of Neurology, Seoul Municipal Boramae Hospital
Department of Neurology, College of Medicine, Seoul National University* |
|
|
|
|
| Abstract |
|
Background : Transient global ischemia causes delayed neuronal death (DND) in the CA1 area, of which the mecha-nism is controversial. Induction of apoptosis-regulating proteins during the process of DND has been reported, howev-er ,the exact role of Bcl-2/Bax is not well understood. We tried to reveal the pattern of the Bax/Bcl-2 expression modi-fied by the duration of ischemia and hypothermia. Methods : Global ischemia was induced in Mongolian gerbils for 2, 5, and 10 minutes under the temperature of 36 ℃and 32 ℃. Hippocampal sections were evaluated 48 hours after ischemia with H&E and immunohistochemical staining to Bcl-2/Bax. Viable neuronal density and semi-quantitative grading were compared. Results : In the CA1 area, neurons were intact in 2 min ischemia, while partial or significant
ischemic changes were observed in 5-10 min ischemia of 36 ℃setting, which were less severe in 32 ℃. Bcl-2 was posi-tive in 2 min ischemia, while negative in 5~10 min ischemia of 36 ℃. Bax was negative in 2 and 10 min ischemia, while positive in 5 min ischemia. In 32 ℃setting, Bcl-2 was also positive in 2 min ischemia and partially positive in 5- 10 min ischemia, although Bax expression was not different from 36 ℃. C o n c l u s I o n s : The complex mechanism of DND, which is in the spectrum of apoptosis and necrosis, seems to be determined by the severity of ischemia. The bal-ance between Bcl-2 and Bax may determine the survival of neurons in mild to moderate ischemia. Further evidence remains to be determined by morphological and molecular biological methods.
J Korean Neurol Assoc 18(4):431~438, 2000
Key Words : Ischemia, Gerbil, Apoptosis, Necrosis, Bax, Bcl-2 |
|
PDF Links
Full text via DOI
Download Citation
