J Korean Neurol Assoc > Volume 17(4); 1999 > Article
Journal of the Korean Neurological Association 1999;17(4): 561-567.
일측 총경동맥을 결찰한 Gerbil 뇌의 초기와 후기의 형태학적 변화
정지향, 구혜수*·최경규
이화여자대학교 의과대학 목동병원 신경과,해부병리과*
Morphological Analysis of Short and Long Term Changes after Ligation of Unilateral Common Carotid Artery in Gerbils
Jee-Hyang Jeong, M.D., Heasoo Koo, M.D.*, Kyung-Gyu Choi, M.D.
Department of Neurology and Pathology*, Ewha Medical Center Mokdong Hospital
Abstract
Background : The ligation of the unilateral common carotid artery (CCA) in the gerbil has been known as an ischemic animal model showing various changes including selective neuronal necrosis as well as infarction. This study was performed to analyze the short and long term morphological changes of transient unilateral forebrain ischemia with special attention to astroglial proliferation. Methods : 67 mongolian gerbils were subjected to 2 hr, 3 hr, 4 hr, or 5 hr of forebrain ischemia by the unilateral CCA ligation method. Each of the ischemic groups were examined after a 1 day, 3 day, or 7 day period of reperfusion. Long term reperfusion groups consisted of 2, 3, and 4 weeks of reperfusion after 5hr of unilateral CCA ligation. Morphological changes were analyzed by H-E staining and an immunohistochemical reaction with GFAP antibody. Results : The ligation of the unilateral CCA, induced unilateral hemispheric infarction in 14 gerbils, selective neuronal necrosis (SNN) involving caudate in 1 gerbil, and delayed neuronal necrosis (DND) of the hippocampal CA1 neurons in 2 gerbils. Infarction was most frequent in 1 day reperfusion groups and did not show any differences according to the duration of ischemia. The GFAP reaction was strongly positive in the center of infarction at a 1 day period and negative at a 3 & 7 day period. The surrounding brain parenchyme progressively revealed increased positive reactions. Gerbils with SNN and DND showed moderately or markedly increased GFAP positive reactions in the unilateral caudate, thalamus, and hippocampus, whereas no apparent changes were shown by a H-E stain. Conclusions : Reactive astrogliosis is a stereotyped reaction of ischemic brain injury and is a more sensitive parameter than neuronal changes. J Kor Neurol Ass 17(4):561~567, 1999 Key Words : Transient Forebrain Ischemia, Astrogliosis, GFAP, Carotid Artery Ligation
TOOLS
METRICS Graph View
  • 1,230 View
  • 1 Download
Related articles

Patterns of Stroke and Collateralization of the Internal Carotid Artery Occlusion in the Neck1998 ;16(4)



ABOUT
BROWSE ARTICLES
EDITORIAL POLICY
FOR CONTRIBUTORS
Editorial Office
(ZIP 03163) #1111, Daeil Bldg, 12, Insadong-gil, Jongno-gu, Seoul, Korea
Tel: +82-2-737-6530    Fax: +82-2-737-6531    E-mail: jkna@neuro.or.kr                

Copyright © 2024 by Korean Neurological Association.

Developed in M2PI

Close layer