Changes of Locomotor Activity and Biogenic Amines by ?1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine in C57BL/6 Mice

J Korean Neurol Assoc > Volume 12(4); 1994 > Article

Journal of the Korean Neurological Association 1994;12(4): 593-603.

1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine이 C57BL/6마우스의 ?행동 및 뇌조직 생체내 아민 변동에 미치는 영향

허지희, 김진수,방혜련,김동구

연세대학교 신경과, 약리학.

Changes of Locomotor Activity and Biogenic Amines by ?1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine in C57BL/6 Mice

Ji Hoe Heo, M.D., Jin Soo Kim, Hye Ryun Bahng, Dong Goo Kim

Department of Neurology, Yonsei University College of Medicine, Department of Pharmacology, Yonsei University College of Medicine

Abstract

The neurotoxicity of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) in rodents was investigated by measuring the locomotor activity and the changes of brain biogenic amines in MPTP-treated C57BL/6 mice. The mice showed a typical curved spine posture 24 hours after MPTP treatment. Total locomotor activity was reduced and the ratios of stereotyped activity/total locomotor activity were increased 24 hours after MPTP treatment. However no significant changes were observed 7 days after MPTP treatment. MPTP-induced changes of biogenic amines were evident only in corpus striatum, not in frontal lobe, midbrain and hippocampus; the levels of dopamine, dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA) were reduced by 94%, 76.3% and 60.2% after 24 hours, and 81.9%, 61.3% and 26.1% after 6 days compared to control values respectively. The ratios of DOPAC/dopamine, HVA/dopamine and HVA/DOPAC were increased 24 hours and 7 day after MPTP treatment compared to control valuse in corpus striatum, but the degree of the 7 days was less than the 24 hours. The ratios of 5-HIAA/5-HT were incresed 24 hours and 7 days after MPTP treatment in corpus striatum, but there were no significant changes in the levels of 5-hydroxyindoleacetic acid (5-HIAA) and 5-hydroxtryptamine (5-HT). In conclusion, MPTP produced parkinsonism-like behavioral and biochemical changes in C57BL/ 6 mice.